Purpose and Goals
- Evaluate and compare the antiviral activity of a/multiple disinfectant products in laboratory-controlled testing.
Testing
- A nonporous glass or stainless steel carrier surface will be inoculated with a liquid viral suspension and dried. The test product, such as an aerosol spray or trigger spray or foam prepared at use-dilution, will then be applied to the carrier surface per the manufacturer’s instructions and incubated at room temperature and ambient relative humidity for a specified contact time. After exposure, the carrier will be recovered into a neutralization solution* which will be dilution-plated in cell cultures to provide quantifiable viable viral burden data for each carrier tested.
* Note: Any disinfectant product requiring gel filtration for complete neutralization will require an additional fee of $1,000 per formulation.
- Testing will include 5% soil (serum) in the inoculum solution.
- Optional: Testing can include residual durability evaluation:
- After the initial viral inoculation and disinfectant application, the carrier surface will be worn with 12 cycles of abrasions (alternating dry and wetted with sterile distilled water) and re-inoculations of viral suspension to simulate 24 hours of wear before recovery into the neutralization solution. Abrasions will be made with the standard Gardco D10V Wear Tester equipment fitted with a weighted abrasion boat of sterilized foam.
- Testing will include the viral strains:
- Influenza A virus (H1N1) ATCC VR-1469
- Human Coronavirus strain OC43 ATCC VR-1558 (a betacoronavirus like SARS-CoV-2)
- Feline calicivirus (FCV) strain F-9 ATCC VR-782 (a surrogate for human norovirus) (a non-enveloped virus)
- Testing will include 72 total data points:
- 3 formulations (such as Formula A, Formula B, and Formula C)
- Note: Please provide enough product for at least 5 mL use-dilution of each formula per data point.
- 2 disinfectant residual durability time-points (0 and 24 hours).
- 4 disinfectant contact time-points (such as 0 minutes, 2 minutes, 5 minutes, and 10 minutes).
- 3 replicate carriers for each test condition
- Note: the number of lots and total replicates is determined by the application submission requirements of the regulatory agency (EPA).
- 3 formulations (such as Formula A, Formula B, and Formula C)
- Note: Optional GLP services are available for an additional fee.
Data Analysis
- In-house control results will be used to verify test validity.
- Data will be calculated to determine the quantifiable viral burden in plaque- or foci-forming units per disc (PFU/disc or FFU/disc) remaining on each test location.
- Data will be analyzed to determine the consistency between replicate swatches.
- Data will be analyzed to determine the percent reductions and log reductions of viral burdens on each/the test carrier compared to the provided negative control at each test condition.
Typical Turn Around Time
- 8 weeks following receipt of the test materials